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Nucleic Acid Quantitation in SolutionSection 8.3 | Thermo , nucleic acid reagent kit principle
Figure 8.3.2 DNA selectivity and sensitivity of the Quant-iT dsDNA high-sensitivity assay. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit ().Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone, the mass of nucleic acid for the RNA alone , nucleic acid reagent kit principleMagNA Pure LC Total Nucleic Acid Isolation KitFor general laboratory use. The MagNA Pure LC Total Nucleic Acid Isolation Kit is designed to isolate high-purity total nucleic acid (e.g., viral DNA and RNA) from mammalian serum, plasma, or whole blood using the MagNA Pure LC Instrument.The purified total nucleic acid can be used in PCR on the LightCycler ® Carousel-Based Systems, the LightCycler ® 480 System, or standard thermal block , nucleic acid reagent kit principleMagNA Pure Compact Nucleic Acid Isolation Kit IThe nucleic acid isolation procedure is based on the proven MagNA Pure Magnetic Glass Particle Technology. The principle steps of a MagNA Pure Compact nucleic acid isolation procedure are: The samples are lysed by incubation with Proteinase K and a special lysis buffer containing a chaotropic salt.
QIAsymphony DSP Circulating DNA Kit - QIAGEN Online Shop
QIAsymphony technology combines the speed and efficiency of anion exchange-based nucleic acid purification with the convenient handling of magnetic particles (see figure Schematic diagram of the QIAsymphony SP principle. The QIAsymphony SP processes a sample containing magnetic particles as follows: A magnetic rod protected by a rod cover enters a well containing the sample and attracts the , nucleic acid reagent kit principleSpin Purification Kits and Reagents | BiocompareSpin Purification Kits and Reagents The spin column method of isolating DNA or RNA is widely used in many nucleic acid purification kits. A type of solid-phase extraction, the principle relies on nucleic acid binding to a solid surface, such as silica gel.QIAamp Circulating Nucleic Acid Kit - QIAGEN Online ShopThe QIAamp Circulating Nucleic Acid Kit efficiently purifies and concentrates free-circulating DNA, RNA, miRNA, and viral nucleic acids from starting materials that contain low concentrations of mostly fragmented DNA and RNA (typically 1100 ng/ml circulating DNA in human plasma). Starting sample volumes can be up to 5 ml.
SARS-CoV-2 Nucleic Acid Test Kit
Please read the entire Instructions carefully before using the kit. 2. This product does not contain nucleic acid extraction reagent, which needs to be prepared by the user. Please refer to [Main Components] for details. 3. The kit can be transported in a foam box with dry ice (or a low temperature ice bag). 4.RNA Reference GuideFigure 1. TRIzol Reagent is the most cited organic reagent for nucleic acid isolation. Citations gathered from 1990 to 2019 according to PubMed records (ncbi.nlm.nih.gov) . Organic extraction RNA reference guideOrganic extraction Homogenize sample in TRIzol Reagent Add chloroform Transfer top aqueous phase to fresh tube Precipitate RNA withNucleic Acid and Protein Quantitation MethodsTo combat the weaknesses of the traditional absorbance nucleic acid concentration method, a variety of fluorescent molecules can also be used for nucleic acid quantitation. The most common of those are Hoechst 33258 and the Life Technologies (Invitrogen) Quant-iT kits. These assays are based on dyes that intercalate into the nucleic acid chain.
Label IT Nucleic Acid Labeling Reagents - Research Reagent , nucleic acid reagent kit principle
Label IT® Nucleic Acid Labeling Kits. The Label IT® chemical labeling reagents are composed of three regions: the label (fluorophore or hapten) (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids and the reactive alkylating group (blue) that covalently attaches the Label IT® reagent to any reactive heteroatom within the nucleic acids.Label IT Nucleic Acid Labeling Reagents - Research Reagent , nucleic acid reagent kit principleLabel IT® Nucleic Acid Labeling Kits. The Label IT® chemical labeling reagents are composed of three regions: the label (fluorophore or hapten) (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids and the reactive alkylating group (blue) that covalently attaches the Label IT® reagent to any reactive heteroatom within the nucleic acids.Nucleic Acid Sample Prep Methods - Beckman CoulterReview Beckman Coulter Life Science's nucleic acid sample prep methods list and supporting applications to find reagent kits for your lab. Beckman Coulter Life Sciences is taking actions in the best interests of our associates, customers, and business partners as we navigate the growing threats of the 2019 Novel Coronavirus disease (COVID-19).
DIG Nucleic Acid Detection Kit | Sigma-Aldrich
DIG Nucleic Acid Detection Kit is used for detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP (alkaline phosphatase) antibody conjugate and the color substrates NBT , nucleic acid reagent kit principleTRIzol RNA Extraction Principle ,Protocol ,Functions of , nucleic acid reagent kit principleDec 23, 2019 · TRIzol Reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components (isoamyl alcohol) which facilitate the extraction of a variety of RNA species of large or small molecular size. It has acidic pH so that the RNA, DNA and proteins get separated in 3 different phases, upper aqueous, interphase and lower , nucleic acid reagent kit principleNucleic Acid Extraction and Purification | LSR | Bio-RadContaminants such as phenol and other organic compounds will absorb at 230 nm. Therefore, another assessment of nucleic acid purity is the absorbance ratio of 260 nm/230 nm. For pure DNA 260 nm/230 nm = ~1.8; For pure RNA 260 nm/230 nm = ~2.0; Integrity The integrity of a nucleic acid sample can be analyzed by gel electrophoresis.
Rapid Kit For HCV Nucleic Acid Detection And Its Detection , nucleic acid reagent kit principle
The hcv rapid test kit, based on the basic reaction principle of conventional rt-qpcr, realizes nucleic acid denaturation at a lower temperature by changing the composition of the reaction buffer and the primer, the unique design and synthesis of the probe, thus shortening the time difference of denaturation and temperature-restoring difference , nucleic acid reagent kit principleEfficacy Assessment of Nucleic Acid Decontamination , nucleic acid reagent kit principleJul 13, 2016 · In this approach equal volumes of the decontamination reagent and the target nucleic acid (i.e. DNA-amplicon or in-vitro RNA) were mixed and incubated for 2 or 10 min, respectively. The reaction was stopped by adding PBS and the lysis buffer intended from the nucleic acid extraction kit.NeuroPorter Transfection Kit Lipid formulation for , nucleic acid reagent kit principleGeneral description Neuroporter Transfection Reagent is a unique formulation of a proprietary cationic lipid optimized for delivery of DNA into primary neurons, glial cells, and cultured neuronal cell lines with high efficiency and low toxicity. The Neuroporter Transfection Kit was designed for difficult-to-transfect primary neurons, addressing past problems such as poor cell viability , nucleic acid reagent kit principle
Nucleic Acid Extraction Methods - BioChain Institute Inc.
Nucleic acid extraction methods are key to molecular biology, and are routinely utilized in many applications in both medical and biological sciences. Nucleic acids were first extracted in 1869 by Friedrich Miescher, when he was studying the chemical nature of the nuclei of white blood cells. In 1953, Rosalind Franklin, James Watson, and , nucleic acid reagent kit principleNucleic Acids The Use of Nucleic Acid Testing in , nucleic acid reagent kit principleIn 1989 Roche entered into an agreement with Cetus to develop diagnostic applications for the novel technique polymerase chain reaction (PCR). A new area of molecular diagnostics began and genes and pathogen genomes have been used to diagnose disease since that point. Automated laboratory platforms were created to facilitate the workflow and allow for accurate and precise processing of patient , nucleic acid reagent kit principleVERIGENE® Respiratory Pathogen Flex Test | Respiratory , nucleic acid reagent kit principleThe VERIGENE® Respiratory Pathogens Flex Test (RP Flex) is an automated, multiplexed, flexible nucleic acid test for the identification of the viruses and bacteria that most commonly cause respiratory infections.. Respiratory testing needs vary by season, geography, and even from patient to patient. Yet currently, to meet diverse testing needs, labs must choose between running expensive mega , nucleic acid reagent kit principle
RNA Purification and Isolation | NEB
For recovery of a specific RNA subpopulation from total RNA derived from cells, tissues or in vitro transcriptions, bead-based methods may be utilized to isolate mRNAs through binding of the poly(A) tail or to isolate RNAs based on size by varying the ratio of nucleic acid to beads in the presence of a crowding reagent. Bead-based methods are a , nucleic acid reagent kit principleLaboratory Procedure ManualThe COBAS® TaqMan® HCV Test, v2.0 is an in vitro nucleic acid amplification test for the quantitation of Hepatitis C Virus (HCV) RNA genotypes 1 through 6 in human serum or plasma, using the COBAS® AmpliPrep Total Nucleic Acid Isolation Kit (TNAI) for theLabCorp COVID-19 RT-PCR test EUA SummaryDEVICE DESCRIPTION AND TEST PRINCIPLE , nucleic acid reagent kit principle Reagent Manufacturer Catalog # , nucleic acid reagent kit principle (3x192 purifications) Roche 06543588001 MagMAX Viral/Pathogen Nucleic Acid Isolation Kit Thermo Fisher A42352 or A48310 , nucleic acid reagent kit principle
VERIGENE® Respiratory Pathogen Flex Test | Respiratory , nucleic acid reagent kit principle
The VERIGENE® Respiratory Pathogens Flex Test (RP Flex) is an automated, multiplexed, flexible nucleic acid test for the identification of the viruses and bacteria that most commonly cause respiratory infections.. Respiratory testing needs vary by season, geography, and even from patient to patient. Yet currently, to meet diverse testing needs, labs must choose between running expensive mega , nucleic acid reagent kit principleRNA Purification and Isolation | NEBFor recovery of a specific RNA subpopulation from total RNA derived from cells, tissues or in vitro transcriptions, bead-based methods may be utilized to isolate mRNAs through binding of the poly(A) tail or to isolate RNAs based on size by varying the ratio of nucleic acid to beads in the presence of a crowding reagent. Bead-based methods are a , nucleic acid reagent kit principleLaboratory Procedure ManualThe COBAS® TaqMan® HCV Test, v2.0 is an in vitro nucleic acid amplification test for the quantitation of Hepatitis C Virus (HCV) RNA genotypes 1 through 6 in human serum or plasma, using the COBAS® AmpliPrep Total Nucleic Acid Isolation Kit (TNAI) for the
LabCorp COVID-19 RT-PCR test EUA Summary
DEVICE DESCRIPTION AND TEST PRINCIPLE , nucleic acid reagent kit principle Reagent Manufacturer Catalog # , nucleic acid reagent kit principle (3x192 purifications) Roche 06543588001 MagMAX Viral/Pathogen Nucleic Acid Isolation Kit Thermo Fisher A42352 or A48310 , nucleic acid reagent kit principleCurrent Nucleic Acid Extraction Methods and Their , nucleic acid reagent kit principleNucleic acid extraction (NAE) plays a vital role in molecular biology as the primary step for many downstream applications. Many modifications have been introduced to the original 1869 method. Modern processes are categorized into chemical or mechanical, each with peculiarities that influence their use, especially in point-of-care diagnostics (POC-Dx).DNA, RNA, and Protein Extraction: The Past and The PresentNov 30, 2009 · An extraction kit based on the principle of magnetic bead based nucleic acid purification is commercially available in the market . The special part of this kit is that the reagents provided are intended for use with magnetic tools.
Nucleic Acid Extraction System ACON Labs
The semi-automatic NES-32 system, developed based on sufficient clinical data, is an efficient and easy to operate nucleic acid extraction system with a throughput of 32 specimens. A variety of specimens such as blood, tissues, body fluids are able to be processed by NES-32 combining with types of magnetic beads extraction reagents.Extraction of DNA using DNAzol Reagent | Thermo Fisher , nucleic acid reagent kit principle6. Quantitation of DNA and Results: Mix an aliquot of solubilized DNA with 1 ml of 8 mM NaOH and measure A 260 and A 280 of the resulting solution. Calculate the DNA content assuming that one A 260 unit equals 50 g of double-stranded DNA per ml.; For calculations of a cell number in analyzed samples or an expected yield of DNA, assume that the amount of DNA per 10 6 diploid cells of human , nucleic acid reagent kit principleUS20060057606A1 - Reagents containing terminal The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time.
Nucleic Acid Extraction Method: The Rise of Magnetic Bead , nucleic acid reagent kit principle
With the rapid development of molecular biology technology, molecular diagnostic and detection technologies represented by nucleic acid hybridization, nucleic acid amplification and nucleic acid sequence analysis have become increasingly important in many fields. As the "first step" of molecular diagnostic experiments, nucleic acid extraction is also the most critical step. The quality of , nucleic acid reagent kit principleRNA Isolation with TRIzol (Invitrogen) and Qiagen methods, regardless of where the nucleic acid was originally extracted. I. PRINCIPLE The Invitrogen Life Technologies TRIzol Reagent (Total RNA Isolation Reagent) is a ready-to-use reagent for the isolation of total RNA from cells and tissues for use in PCR analysis. TRIzol reagent is a mono-phasic solution of phenol and guanidine isothiocyanate.GeneXpert MTB/RIF Assay: Principle, Procedure, Results GeneXpert MTB/RIF assay is a nucleic acid amplification (NAA) test which simultaneously detects DNA of Mycobacterium tuberculosis complex (MTBC) and resistance to rifampin (RIF) (i.e. mutation of the rpoB gene) in less than 2 hours. In comparison, standard cultures can take 2 to 6 weeks for MTBC to grow and conventional drug resistance tests , nucleic acid reagent kit principle
US5476769A - Method for assays of nucleic acid, a reagent , nucleic acid reagent kit principle
US5476769A US07/610,470 US61047090A US5476769A US 5476769 A US5476769 A US 5476769A US 61047090 A US61047090 A US 61047090A US 5476769 A US5476769 A Simple amplification-based assay: A nucleic acid-based , nucleic acid reagent kit principleVisual detection of nucleic acid by dipstick in the simple amplification-based assay (SAMBA) format. A, Principle of the signal amplification system (SAS) for detection of nucleic acid. The SAS is based on detector probes labeled with multiple hapten moieties that form large lattice-like structures by specifically binding to anti-hapten , nucleic acid reagent kit principle